Glicoproteínas/metabolismo; Lactose/análogos & derivados; Proteínas do Leite/metabolismo; Ácido N-Acetilneuramínico/metabolismo; Neuraminidase/metabolismo; Oligossacarídeos/metabolismo; Pasteurella multocida/enzimologia; Sialiltransferases/metabolismo; Proteínas de Bactérias/metabolismo; Humanos; Concentração de Íons de Hidrogênio; Lactose/metabolismo; Proteínas Recombinantes/metabolismo; Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz; Temperatura; Proteínas do Soro do Leite

2-O-(p-nitrophenyl)-α-d-N-acetylneuraminic acid; 4-methylumbelliferyl ß-d-lactoside; ATP; CMP-NeuAc; CTP; CV; Casein glycomacropeptide as source for sialic acid; Enzyme expression and purification; GOS; GalNAc; HMOs; HPAEC-PAD; Human milk oligosaccharides; Kinetics of trans-sialidase; LC­MS; LacMu; MALDI-MS; N-His6-tag; N-acetylgalactosamine; N-acetylneuraminic acid; N-terminal His(6)-tag; NDV; Neu5Ac; Newcastle disease virus; PEP; PGC; Pasteurella multocida sialyltransferase; PmST; RSM; Response surface; SDS-PAGE; Sia-GOS; TLC; adenosine-5'-triphosphate; cGMP; capillary liquid chromatography/mass spectrometry; casein glycomacropeptide; column volume; cytidine-5'-monophospho-N-acetylneuraminic acid; cytidine-5'-triphosphate; galactooligosaccharides; high-performance anion exchange chromatography; human milk oligosaccharides; matrix-assisted laser desorption/ionization mass spectrometry; pNP-Neu5Ac; phosphoenolpyruvate; porous graphitic carbon; response surface methodology; sialylated galactooligosaccharides; sodium dodecyl sulfate polyacrylamide gel electrophoresis; thin-layer chromatography