Simultaneous quantitation and validation of triterpenoids and phytosteroids in Phaseolus angularis seeds.
Molecules
; 19(7): 10309-19, 2014 Jul 16.
Article
em En
| MEDLINE
| ID: mdl-25033058
ABSTRACT
A reproducible analytical method using reverse-phase high liquid performance chromatography combined with UV detecting was developed for the quantitative determination of four compounds isolated from the ethanol extract of Phaseolus angularis seeds (PASE) oleanolic acid (1), oleanolic acid acetate (2), stigmasterol (3) and ß-sitosterol (4). This method was fully validated in terms of linearity (r2 > 0.999), accuracy (98.5%-100.8%), precision (<0.92%), LOD (<0.0035 mg/mL), and LOQ (<0.0115 mg/mL). The effects of the PASE and isolated compounds 1-4 on TLR4 activation were tested in THP1-Blue cells. Among the tested substances, compound 2 showed potent inhibitory activity with an IC50 value of 3.89 ± 0.17 µM.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article