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Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser.
Arnlund, David; Johansson, Linda C; Wickstrand, Cecilia; Barty, Anton; Williams, Garth J; Malmerberg, Erik; Davidsson, Jan; Milathianaki, Despina; DePonte, Daniel P; Shoeman, Robert L; Wang, Dingjie; James, Daniel; Katona, Gergely; Westenhoff, Sebastian; White, Thomas A; Aquila, Andrew; Bari, Sadia; Berntsen, Peter; Bogan, Mike; van Driel, Tim Brandt; Doak, R Bruce; Kjær, Kasper Skov; Frank, Matthias; Fromme, Raimund; Grotjohann, Ingo; Henning, Robert; Hunter, Mark S; Kirian, Richard A; Kosheleva, Irina; Kupitz, Christopher; Liang, Mengning; Martin, Andrew V; Nielsen, Martin Meedom; Messerschmidt, Marc; Seibert, M Marvin; Sjöhamn, Jennie; Stellato, Francesco; Weierstall, Uwe; Zatsepin, Nadia A; Spence, John C H; Fromme, Petra; Schlichting, Ilme; Boutet, Sébastien; Groenhof, Gerrit; Chapman, Henry N; Neutze, Richard.
Afiliação
  • Arnlund D; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Johansson LC; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Wickstrand C; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Barty A; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany.
  • Williams GJ; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Malmerberg E; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Davidsson J; Department of Chemistry - Ångström Laboratory, Uppsala University, Uppsala, Sweden.
  • Milathianaki D; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • DePonte DP; 1] Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany. [2] Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Shoeman RL; 1] Max-Planck-Institut für medizinische Forschung, Heidelberg, Germany. [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Hamburg, Germany.
  • Wang D; Department of Physics, Arizona State University, Tempe, Arizona, USA.
  • James D; Department of Physics, Arizona State University, Tempe, Arizona, USA.
  • Katona G; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Westenhoff S; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • White TA; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany.
  • Aquila A; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany.
  • Bari S; 1] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Hamburg, Germany. [2] Max-Planck-Institut für Kernphysik, Heidelberg, Germany.
  • Berntsen P; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Bogan M; PULSE Institute for Ultrafast Energy Science, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • van Driel TB; Department of Physics, Technical University of Denmark, Lyngby, Denmark.
  • Doak RB; 1] Max-Planck-Institut für medizinische Forschung, Heidelberg, Germany. [2] Department of Physics, Arizona State University, Tempe, Arizona, USA.
  • Kjær KS; 1] Department of Physics, Technical University of Denmark, Lyngby, Denmark. [2] Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark.
  • Frank M; Lawrence Livermore National Laboratory, Livermore, California, USA.
  • Fromme R; Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA.
  • Grotjohann I; Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA.
  • Henning R; BioCARS, University of Chicago, Chicago, Illinois, USA.
  • Hunter MS; Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA.
  • Kirian RA; Department of Physics, Arizona State University, Tempe, Arizona, USA.
  • Kosheleva I; BioCARS, University of Chicago, Chicago, Illinois, USA.
  • Kupitz C; Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA.
  • Liang M; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany.
  • Martin AV; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany.
  • Nielsen MM; Department of Physics, Technical University of Denmark, Lyngby, Denmark.
  • Messerschmidt M; 1] Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany. [2] Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Seibert MM; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Sjöhamn J; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Stellato F; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany.
  • Weierstall U; Department of Physics, Arizona State University, Tempe, Arizona, USA.
  • Zatsepin NA; Department of Physics, Arizona State University, Tempe, Arizona, USA.
  • Spence JC; Department of Physics, Arizona State University, Tempe, Arizona, USA.
  • Fromme P; Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA.
  • Schlichting I; 1] Max-Planck-Institut für medizinische Forschung, Heidelberg, Germany. [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Hamburg, Germany.
  • Boutet S; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Groenhof G; 1] Nanoscience Center, University of Jyväskylä, Jyväskylä, Finland. [2] Department of Chemistry, University of Jyväskylä, Jyväskylä, Finland.
  • Chapman HN; 1] Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany. [2] Department of Physics, University of Hamburg, Hamburg, Germany. [3] Centre for Ultrafast Imaging, Hamburg, Germany.
  • Neutze R; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
Nat Methods ; 11(9): 923-6, 2014 Sep.
Article em En | MEDLINE | ID: mdl-25108686
ABSTRACT
We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake' the hypothesis that proteins rapidly dissipate energy through quake-like structural motions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2014 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2014 Tipo de documento: Article