Your browser doesn't support javascript.
loading
The Streptococcus mutans irvA gene encodes a trans-acting riboregulatory mRNA.
Liu, Nan; Niu, Guoqing; Xie, Zhoujie; Chen, Zhiyun; Itzek, Andreas; Kreth, Jens; Gillaspy, Allison; Zeng, Lin; Burne, Robert; Qi, Fengxia; Merritt, Justin.
Afiliação
  • Liu N; Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 71304, USA.
  • Niu G; Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 71304, USA.
  • Xie Z; Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 71304, USA.
  • Chen Z; Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 71304, USA.
  • Itzek A; Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 71304, USA.
  • Kreth J; Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 71304, USA; Division of Oral Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
  • Gillaspy A; Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 71304, USA.
  • Zeng L; Department of Oral Biology, University of Florida, Gainesville, FL 32610, USA.
  • Burne R; Department of Oral Biology, University of Florida, Gainesville, FL 32610, USA.
  • Qi F; Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 71304, USA; Division of Oral Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
  • Merritt J; Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 71304, USA; Division of Oral Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. Electronic address: justin-merritt@ouhsc.edu.
Mol Cell ; 57(1): 179-90, 2015 Jan 08.
Article em En | MEDLINE | ID: mdl-25574948
In both prokaryotes and eukaryotes, insight into gene function is typically obtained by in silico homology searches and/or phenotypic analyses of strains bearing mutations within open reading frames. However, the studies herein illustrate how mRNA function is not limited to the expression of a cognate protein. We demonstrate that a stress-induced protein-encoding mRNA (irvA) from the dental caries pathogen Streptococcus mutans directly modulates target mRNA (gbpC) stability through seed pairing interactions. The 5' untranslated region of irvA mRNA is a trans riboregulator of gbpC and a critical activator of the DDAG stress response, whereas IrvA functions independently in the regulation of natural competence. The irvA riboregulatory domain controls GbpC production by forming irvA-gbpC hybrid mRNA duplexes that prevent gbpC degradation by an RNase J2-mediated pathway. These studies implicate a potentially ubiquitous role for typical protein-encoding mRNAs as riboregulators, which could alter current concepts in gene regulation.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2015 Tipo de documento: Article