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Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis.
Dreyfuss, Juliana L; Regatieri, Caio V; Coelho, Bruno; Barbosa, José B; De Freitas, Denise; Nader, Helena B; Martins, João R.
Afiliação
  • Dreyfuss JL; Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
  • Regatieri CV; Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
  • Coelho B; Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
  • Barbosa JB; Departamento de Oftalmologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
  • De Freitas D; Departamento de Oftalmologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
  • Nader HB; Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
  • Martins JR; Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
An Acad Bras Cienc ; 87(1): 455-62, 2015 Mar.
Article em En | MEDLINE | ID: mdl-25714075
The adenoviral conjunctivitis is one of the biggest causes of conjunctival infection in the world. Conjunctivitis causes relatively nonspecific symptoms, as hyperaemia and chemosis. Even after biomicroscopy, complex laboratory tests, such as viral culture, are necessary to identify the pathogen or its etiology. To contribute to the better understanding of the pathobiology of the adenoviral conjunctivitis, the tear fluids of patients with unilateral acute adenovirus conjunctivitis (UAAC), normal donors (control) and patients with allergic conjunctivitis were analyzed. Tear samples were collected with Schirmer strips from control, allergic conjunctivitis and UAAC patients, diagnosed by clinical signs. UAAC tears were tested positive in viral cultures. After the elution, HA was quantified using an ELISA-like fluorometric assay and the protein profile was determined by SDS-PAGE. A profound increase in the HA tear content in UAAC patients was found when compared to control and ALC. This HA increase in UAAC tears remarkably was not observed in tears from contralateral eyes without clinical signs, nor in allergic conjunctivitis. In addition a distinct profile of UAAC tear proteins was observed in patients with UAAC. The quantification of HA in the tear fluid is a rapid, sensitive and specific test. This molecule might be a biomarker candidate for acute conjunctivitis.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Observational_studies / Prognostic_studies Limite: Adult / Child / Female / Humans / Male Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Observational_studies / Prognostic_studies Limite: Adult / Child / Female / Humans / Male Idioma: En Ano de publicação: 2015 Tipo de documento: Article