Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage.
Front Microbiol
; 6: 316, 2015.
Article
em En
| MEDLINE
| ID: mdl-25941520
ABSTRACT
F-specific filamentous phage of Escherichia coli (Ff f1, M13, or fd) are long thin filaments (860 nm × 6 nm). They have been a major workhorse in display technologies and bionanotechnology; however, some applications are limited by the high length-to-diameter ratio of Ff. Furthermore, use of functionalized Ff outside of laboratory containment is in part hampered by the fact that they are genetically modified viruses. We have now developed a system for production and purification of very short functionalized Ff-phage-derived nanorods, named Ff-nano, that are only 50 nm in length. In contrast to standard Ff-derived vectors that replicate in E. coli and contain antibiotic-resistance genes, Ff-nano are protein-DNA complexes that cannot replicate on their own and do not contain any coding sequences. These nanorods show an increased resistance to heating at 70(∘)C in 1% SDS in comparison to the full-length Ff phage of the same coat composition. We demonstrate that functionalized Ff-nano particles are suitable for application as detection particles in sensitive and quantitative "dipstick" lateral flow diagnostic assay for human plasma fibronectin.
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01-internacional
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MEDLINE
Idioma:
En
Ano de publicação:
2015
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Article