Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries.
Malar J
; 14: 233, 2015 Jun 04.
Article
em En
| MEDLINE
| ID: mdl-26040274
ABSTRACT
BACKGROUND:
The chemical treatment of Plasmodium falciparum for human infections is losing efficacy each year due to the rise of resistance. One possible strategy to find novel anti-malarial drugs is to access the largest reservoir of genomic biodiversity source on earth present in metagenomes of environmental microbial communities.METHODS:
A bioluminescent P. falciparum parasite was used to quickly detect shifts in viability of microcultures grown in 96-well plates. A synthetic gene encoding the Dermaseptin 4 peptide was designed and cloned under tight transcriptional control in a large metagenomic insert context (30 kb) to serve as proof-of-principle for the screening platform.RESULTS:
Decrease in parasite viability consistently correlated with bioluminescence emitted from parasite microcultures, after their exposure to bacterial extracts containing a plasmid or fosmid engineered to encode the Dermaseptin 4 anti-malarial peptide.CONCLUSIONS:
Here, a new technical platform to access the anti-malarial potential in microbial environmental metagenomes has been developed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article