Monitoring of phosphorylated peptides by radioactive assay and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry.

Kang, Jeong-Hun; Asai, Daisuke; Toita, Riki; Kawano, Takahito; Murata, Masaharu

Kang, Jeong-Hun; Asai, Daisuke; Toita, Riki; Kawano, Takahito; Murata, Masaharu.

Afiliação

Kang JH; Division of Biopharmaceutics and Pharmacokinetics, Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, 5-7-1, Fujishirodai, Suita, Osaka, 565-8565, Japan. jrjhkang@ncvc.go.jp.
Asai D; Department of Microbiology, St. Marianna University School of Medicine, Sugao 2-16-1 Miyamae, Kawasaki, 216-8511, Japan.
Toita R; Department of Biomaterials, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Kawano T; Department of Advanced Medical Initiatives, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Murata M; Department of Advanced Medical Initiatives, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.

Amino Acids ; 47(11): 2377-83, 2015 Nov.

Article em En | MEDLINE | ID: mdl-26077716

Assuntos

Peptídeos/química; Fosfoproteínas/química; Radioisótopos de Fósforo/química; Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos; Animais; Quinase 2 de Receptor Acoplado a Proteína G/química; Humanos; Fosforilação

Palavras-chave

Counts per minute; MALDI-TOF-MS; Negative mode; Phosphorylation; Protein kinase; Radioactive assay

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article