The first complete chloroplast genome sequence from Violaceae (Viola seoulensis).

The complete chloroplast genome of Viola seoulensis, an endemic species to Korea, was determined in this study. The total genome size was 156 507 bp in length, containing a pair of inverted repeats (IRs) of 26 404 bp, which were separated by large single copy (LSC) and small single copy (SSC) of 85 691 and 18 008 bp, respectively. The overall GC contents of the plastid genome were 36.3%. One hundred and thirty genes were annotated, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. In these genes, 17 genes contained one or two introns. A phylogenetic tree showed that Violaceae was closely related to Salicaceae.