Temperature-absorption transition spectroscopy: a technique for estimating the relative charge densities on thiolated phosphate groups in nucleic acid analogues.

Various polynucleotides with well-defined sequences at low salt concentration have been studied using temperature-absorbtion transition spectroscopy to understand their conformations. It has been observed that the adopted technique can fairly estimate the relative charge densities at the thiolated phosphate groups in nucleic acid analogues at low salt concentrations.