Polymorphisms of folate metabolism genes in patients with cirrhosis and hepatocellular carcinoma.
World J Hepatol
; 8(29): 1234-1243, 2016 Oct 18.
Article
em En
| MEDLINE
| ID: mdl-27803768
ABSTRACT
AIM:
To evaluated the association of the risk factors and polymorphisms in MTHFR C677T, MTHFR A1298C, MTR A2756G and MTRR A66G genes.METHODS:
Patients with cirrhosis (n = 116), hepatocellular carcinoma (HCC) (n = 71) and controls (n = 356) were included. Polymerase chain reaction followed by enzymatic digestion and allelic discrimination technique real-time PCR techniques were used for analysis. MINITAB-14.0 and SNPstats were utilized for statistical analysis.RESULTS:
Showed that age ≥ 46 years (OR = 10.31; 95%CI 5.66-18.76; P < 0.001) and smoking (OR = 0.47; 95%CI 0.28-0.78; P = 0.003) were associated with cirrhosis. Age ≥ 46 years (OR = 16.36; 95%CI 6.68-40.05; P < 0.001) and alcohol habit (OR = 2.01; 95%CI 1.03-3.89; P = 0.039) were associated with HCC. MTHFR A1298C in codominant model (OR = 3.37; 95%CI 1.52-7.50; P = 0.014), recessive model (OR = 3.04; 95%CI 1.43-6.47; P = 0.0051) and additive model (OR = 1.71; 95%CI 1.16-2.52; P = 0.0072) was associated with HCC, as well as MTR A2756G in the additive model (OR = 1.68; 95%CI 1.01-2.77; P = 0.047), and MTRR A66G in the codominant model (OR = 3.26; 95%CI 1.54-6.87; P < 0.001), dominant model (OR = 2.55; 95%CI 1.24-5.25; P = 0.007) and overdominant model (OR = 3.05; 95%CI 1.66-5.62; P < 0.001). MTR A2756G in the additive model (OR = 1.54; 95%CI 1.02-2.33; P = 0.042) and smokers who presented at least one polymorphic allele for MTRR A66G (OR = 1.71; 95%CI 0.77-3.82; P = 0.0051) showed increased risk for cirrhosis. There was no association between clinical parameters and polymorphisms.CONCLUSION:
Age ≥ 46 years, alcohol habit and MTR A2756G, MTHFR A1298C and MTRR A66G polymorphisms are associated with an increased risk of HCC development; age ≥ 46 years, tobacco habit and the MTR A2756G polymorphism are associated with cirrhosis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article