Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9.
Stem Cell Res
; 17(2): 441-443, 2016 09.
Article
em En
| MEDLINE
| ID: mdl-27879221
ABSTRACT
Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The heterozygous knockout cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article