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Synthetic Uncleavable Ubiquitinated Proteins Dissect Proteasome Deubiquitination and Degradation, and Highlight Distinctive Fate of Tetraubiquitin.
Singh, Sumeet K; Sahu, Indrajit; Mali, Sachitanand M; Hemantha, Hosahalli P; Kleifeld, Oded; Glickman, Michael H; Brik, Ashraf.
Afiliação
  • Singh SK; Schulich Faculty of Chemistry, Technion-Israel Institute of Technology , 3200008 Haifa, Israel.
  • Sahu I; Department of Biology Technion-Israel Institute of Technology , 3200008 Haifa, Israel.
  • Mali SM; Schulich Faculty of Chemistry, Technion-Israel Institute of Technology , 3200008 Haifa, Israel.
  • Hemantha HP; Schulich Faculty of Chemistry, Technion-Israel Institute of Technology , 3200008 Haifa, Israel.
  • Kleifeld O; Department of Biology Technion-Israel Institute of Technology , 3200008 Haifa, Israel.
  • Glickman MH; Department of Biology Technion-Israel Institute of Technology , 3200008 Haifa, Israel.
  • Brik A; Schulich Faculty of Chemistry, Technion-Israel Institute of Technology , 3200008 Haifa, Israel.
J Am Chem Soc ; 138(49): 16004-16015, 2016 12 14.
Article em En | MEDLINE | ID: mdl-27960333
Various hypotheses have been proposed regarding how chain length, linkage type, position on substrate, and susceptibility to deubiquitinases (DUBs) affect processing of different substrates by proteasome. Here we report a new strategy for the chemical synthesis of ubiquitinated proteins to generate a set of well-defined conjugates bearing an oxime bond between the chain and the substrate. We confirmed that this isopeptide replacement is resistant to DUBs and to shaving by proteasome. Analyzing products generated by proteasomes ranked how chain length governed degradation outcome. Our results support that (1) the cleavage of the proximal isopeptide bond is not a prerequisite for proteasomal degradation, (2) by overcoming trimming at the proteasome, tetraUb is a fundamentally different signal than shorter chains, and (3) the tetra-ubiquitin chain can be degraded with the substrate. Together these results highlight the usefulness of chemistry to dissect the contribution of proteasome-associated DUBs and the complexity of the degradation process.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article