An efficient and simple method to increase the level of displayed protein on the yeast cell surface.

Zhao, Jing-Zhuang; Xu, Li-Ming; Liu, Miao; Cao, Yong-Sheng; LaPatra, Scott E; Yin, Jia-Sheng; Liu, Hong-Bai; Lu, Tong-Yan

Zhao, Jing-Zhuang; Xu, Li-Ming; Liu, Miao; Cao, Yong-Sheng; LaPatra, Scott E; Yin, Jia-Sheng; Liu, Hong-Bai; Lu, Tong-Yan.

Afiliação

Zhao JZ; Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: zhaojingzhuang1987@163.com.
Xu LM; Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: lmxu0917@163.com.
Liu M; Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: 446048847@qq.com.
Cao YS; Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: 375825652@qq.com.
LaPatra SE; Clear Springs Foods Inc., Research Division, PO Box 712, Buhl, ID 83316, USA. Electronic address: scott.lapatra@clearsprings.com.
Yin JS; Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: xwsc20@tom.com.
Liu HB; Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: 112893129@qq.com.
Lu TY; Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: lutongyan@hrfri.ac.cn.

J Microbiol Methods ; 135: 41-47, 2017 04.

Article em En | MEDLINE | ID: mdl-28188810

Assuntos

Antígenos de Superfície; Técnicas de Visualização da Superfície Celular/métodos; Proteínas Fúngicas/metabolismo; Saccharomyces cerevisiae/metabolismo; Anticorpos; Antígenos de Superfície/genética; Antígenos de Superfície/metabolismo; Western Blotting/métodos; DNA Bacteriano; DNA Fúngico; DNA Recombinante; Escherichia coli/genética; Escherichia coli/metabolismo; Citometria de Fluxo/métodos; Proteínas Fúngicas/imunologia; Regulação Fúngica da Expressão Gênica; Microscopia de Fluorescência/métodos; Anticorpos de Cadeia Única/metabolismo; Vacinas

Palavras-chave

Display capacity; Display method; Saccharomyces cerevisiae; Surface display technology; Yeast surface display

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2017 Tipo de documento: Article