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A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid.
Tálas, András; Kulcsár, Péter István; Weinhardt, Nóra; Borsy, Adrienn; Tóth, Eszter; Szebényi, Kornélia; Krausz, Sarah Laura; Huszár, Krisztina; Vida, István; Sturm, Ádám; Gordos, Bianka; Hoffmann, Orsolya Ivett; Bencsura, Petra; Nyeste, Antal; Ligeti, Zoltán; Fodor, Elfrieda; Welker, Ervin.
Afiliação
  • Tálas A; School of Ph.D. Studies, Semmelweis University, Budapest, Hungary.
  • Kulcsár PI; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary.
  • Weinhardt N; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary.
  • Borsy A; Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
  • Tóth E; University of Szeged, Szeged, Hungary.
  • Szebényi K; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary.
  • Krausz SL; Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
  • Huszár K; University of Szeged, Szeged, Hungary.
  • Vida I; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary.
  • Sturm Á; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary.
  • Gordos B; Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
  • Hoffmann OI; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary.
  • Bencsura P; School of Ph.D. Studies, Semmelweis University, Budapest, Hungary.
  • Nyeste A; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary.
  • Ligeti Z; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary.
  • Fodor E; Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
  • Welker E; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary.
DNA Res ; 24(6): 609-621, 2017 Dec 01.
Article em En | MEDLINE | ID: mdl-28679166
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals / Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals / Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article