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Protein corona and phospholipase activity drive selective accumulation of nanomicelles in atherosclerotic plaques.
Lechuga-Vieco, Ana V; Groult, Hugo; Pellico, Juan; Mateo, Jesús; Enríquez, Jose A; Ruiz-Cabello, Jesús; Herranz, Fernando.
Afiliação
  • Lechuga-Vieco AV; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) and CIBERES, Madrid, Spain.
  • Groult H; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) and CIBERES, Madrid, Spain; Université de La Rochelle, UMR 7266 CNRS-ULR, LIENSS, Faculté des Sciences et Technologies, Bâtiment Marie Curie, La Rochelle, France.
  • Pellico J; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) and CIBERES, Madrid, Spain.
  • Mateo J; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) and CIBERES, Madrid, Spain.
  • Enríquez JA; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) and CIBERES, Madrid, Spain.
  • Ruiz-Cabello J; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) and CIBERES, Madrid, Spain; Departamento Química Física II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain. Electronic address: ruizcabe@ucm.es.
  • Herranz F; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) and CIBERES, Madrid, Spain. Electronic address: fherranz@cnic.es.
Nanomedicine ; 14(3): 643-650, 2018 04.
Article em En | MEDLINE | ID: mdl-29317346
ApoB-100 and Phosphatidylcholine-specific phospholipase C (PC-PLC) are important contributors to atherosclerosis development. ApoB-100 is the main structural protein of LDL, being directly associated with atherosclerosis plaque generation. PC-PLC is highly expressed in atherosclerosis lesions and contributes to their progression. We show how phosphatidylcholine-coated nanomicelles can be used for specific characterisation of atherosclerosis plaque. Results show that ApoB-100 in the protein corona of the nanomicelle targets the particles to atherosclerotic areas in apolipoprotein E-/- mice. Furthermore, PC-PLC selectively removes the polar heads from the phospholipid coating of the nanomicelles leading to their accumulation. To fully characterise the behaviour of the nanomicelles, we developed multimodal probes using a nanoemulsion step. Hybrid imaging revealed plaque accumulation of the nanomicelles and colocalisation with PC-PLC expression and ApoB-100 in the plaque. This study shows how protein corona composition and enzyme-driven nanomaterial accumulation can be used for detection of atherosclerosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article