Inhibition of HSV-2 infection by pure compounds from Thymus capitatus extract in vitro.
Phytother Res
; 32(8): 1555-1563, 2018 08.
Article
em En
| MEDLINE
| ID: mdl-29673001
ABSTRACT
Thymus capitatus represents 1 of the 5 Tunisian species of the genus Thymus, which has longstanding use for flavouring and preserving several food products. Its constituents have been reported to endow antimicrobial properties, but little is known about their antiviral activities. The aim of this study was to examine the antiviral activity of pure compounds from the most bioactive inhibitory T. capitatus extract in vitro against herpes simplex virus Type 2 (HSV2) infection and to identify their mechanism of action. Either the extracts or the essential oil exert inhibitory activity against HSV2 infection, with the ethanolic extract showing the lowest EC50 value (2.3 µg/ml). Three pure compounds were then isolated from the ethanolic extract and investigated for their antiviral activity. ßsitosterol showed the most favourable selectivity index and both cinnamaldehyde and carvacrol exerted moderate antiviral effect. Investigation of the mechanism of action revealed that all three compounds directly inactivated the infectivity of the virus particles. These findings suggest the use of T. capitatus ethanolic extract as source of antiHSV2 pure compounds and warrant further studies to evaluate their therapeutic potential.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article