Enhanced N-acetyl-D-neuraminic production from glycerol and N-acetyl-D-glucosamine by metabolically engineered Escherichia coli with a two-stage pH-shift control strategy.

Zhu, De-Qiang; Wu, Jian-Rong; Zhan, Xiao-Bei; Zhu, Li; Jiang, Yun

Zhu, De-Qiang; Wu, Jian-Rong; Zhan, Xiao-Bei; Zhu, Li; Jiang, Yun.

Afiliação

Zhu DQ; Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Wu JR; State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China.
Zhan XB; Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Zhu L; Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China. xbzhan@yahoo.com.
Jiang Y; Jiangsu Rayguang Biotech Co. Ltd., Wuxi, 214122, China.

J Ind Microbiol Biotechnol ; 46(2): 125-132, 2019 Feb.

Article em En | MEDLINE | ID: mdl-30623269

Assuntos

Escherichia coli/genética; Microrganismos Geneticamente Modificados/genética; Ácido N-Acetilneuramínico/biossíntese; Acetilglucosamina/metabolismo; Técnicas de Cultura Celular por Lotes; Biocatálise; Escherichia coli/metabolismo; Glicerol/metabolismo; Concentração de Íons de Hidrogênio; Engenharia Metabólica; Ácido Pirúvico/metabolismo

Palavras-chave

Biocatalytic process; Energy source; Fed-batch; Glycerol; N-Acetyl-D-neuraminic acid

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article