Biophysical Characterization of a Disabled Double Mutant of Soybean Lipoxygenase: The "Undoing" of Precise Substrate Positioning Relative to Metal Cofactor and an Identified Dynamical Network.

Hu, Shenshen; Offenbacher, Adam R; Thompson, Erin M; Gee, Christine L; Wilcoxen, Jarett; Carr, Cody A M; Prigozhin, Daniil M; Yang, Vanessa; Alber, Tom; Britt, R David; Fraser, James S; Klinman, Judith P

Hu, Shenshen; Offenbacher, Adam R; Thompson, Erin M; Gee, Christine L; Wilcoxen, Jarett; Carr, Cody A M; Prigozhin, Daniil M; Yang, Vanessa; Alber, Tom; Britt, R David; Fraser, James S; Klinman, Judith P.

Afiliação

Offenbacher AR; Department of Chemistry , East Carolina University , Greenville , North Carolina 27858 , United States.
Thompson EM; Department of Bioengineering and Therapeutic Science , University of California, San Francisco , San Francisco , California 94158 , United States.
Wilcoxen J; Department of Chemistry , University of California, Davis , Davis , California 95695 , United States.
Britt RD; Department of Chemistry , University of California, Davis , Davis , California 95695 , United States.
Fraser JS; Department of Bioengineering and Therapeutic Science , University of California, San Francisco , San Francisco , California 94158 , United States.

J Am Chem Soc ; 141(4): 1555-1567, 2019 01 30.

Article em En | MEDLINE | ID: mdl-30645119

Assuntos

Coenzimas/metabolismo; Glycine max/enzimologia; Lipoxigenase/química; Lipoxigenase/metabolismo; Metais/metabolismo; Mutação; Domínio Catalítico; Cinética; Lipoxigenase/genética; Modelos Moleculares; Oxirredução; Termodinâmica

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article