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Wheat ms5 male-sterility is induced by recessive homoeologous A and D genome non-specific lipid transfer proteins.
Pallotta, Margaret A; Warner, Patricia; Kouidri, Allan; Tucker, Elise J; Baes, Mathieu; Suchecki, Radoslaw; Watson-Haigh, Nathan; Okada, Takashi; Garcia, Melissa; Sandhu, Ajay; Singh, Manjit; Wolters, Petra; Albertsen, Marc C; Cigan, A Mark; Baumann, Ute; Whitford, Ryan.
Afiliação
  • Pallotta MA; School of Agriculture, Food & Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
  • Warner P; School of Agriculture, Food & Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
  • Kouidri A; School of Agriculture, Food & Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
  • Tucker EJ; School of Agriculture, Food & Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
  • Baes M; School of Agriculture, Food & Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
  • Suchecki R; School of Agriculture, Food & Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
  • Watson-Haigh N; School of Agriculture, Food & Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
  • Okada T; School of Agriculture, Food & Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
  • Garcia M; School of Agriculture, Food & Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
  • Sandhu A; DuPont Pioneer Hi-Bred International Inc., 7250 NW 62nd Avenue, Johnston, IA, 50131-0552, USA.
  • Singh M; DuPont Pioneer Hi-Bred International Inc., 7250 NW 62nd Avenue, Johnston, IA, 50131-0552, USA.
  • Wolters P; DuPont Pioneer Hi-Bred International Inc., 7250 NW 62nd Avenue, Johnston, IA, 50131-0552, USA.
  • Albertsen MC; DuPont Pioneer Hi-Bred International Inc., 7250 NW 62nd Avenue, Johnston, IA, 50131-0552, USA.
  • Cigan AM; DuPont Pioneer Hi-Bred International Inc., 7250 NW 62nd Avenue, Johnston, IA, 50131-0552, USA.
  • Baumann U; School of Agriculture, Food & Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
  • Whitford R; School of Agriculture, Food & Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
Plant J ; 99(4): 673-685, 2019 08.
Article em En | MEDLINE | ID: mdl-31009129
Nuclear male-sterile mutants with non-conditional, recessive and strictly monogenic inheritance are useful for both hybrid and conventional breeding systems, and have long been a research focus for many crops. In allohexaploid wheat, however, genic redundancy results in rarity of such mutants, with the ethyl methanesulfonate-induced mutant ms5 among the few reported to date. Here, we identify TaMs5 as a glycosylphosphatidylinositol-anchored lipid transfer protein required for normal pollen exine development, and by transgenic complementation demonstrate that TaMs5-A restores fertility to ms5. We show ms5 locates to a centromere-proximal interval and has a sterility inheritance pattern modulated by TaMs5-D but not TaMs5-B. We describe two allelic forms of TaMs5-D, one of which is non-functional and confers mono-factorial inheritance of sterility. The second form is functional but shows incomplete dominance. Consistent with reduced functionality, transcript abundance in developing anthers was found to be lower for TaMs5-D than TaMs5-A. At the 3B homoeolocus, we found only non-functional alleles among 178 diverse hexaploid and tetraploid wheats that include landraces and Triticum dicoccoides. Apparent ubiquity of non-functional TaMs5-B alleles suggests loss-of-function arose early in wheat evolution and, therefore, at most knockout of two homoeoloci is required for sterility. This work provides genetic information, resources and tools required for successful implementation of ms5 sterility in breeding systems for bread and durum wheats.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2019 Tipo de documento: Article