Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes.
Methods Mol Biol
; 2061: 13-24, 2020.
Article
em En
| MEDLINE
| ID: mdl-31583649
ABSTRACT
Here we present an optimized protocol for immunolocalization of meiotic proteins during female meiosis in whole mount tissues. It ensures ovule morphology integrity and homogeneous reagent penetration. The method relies on paraformaldehyde tissue fixation, polyacrylamide embedding, tissue permeabilization, antibody incubation, counterstaining, and confocal microscopy analysis. This protocol has been used in diverse Arabidopsis ecotypes and in the legume Vigna unguiculata.
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Coleções:
01-internacional
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MEDLINE
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article