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Oxidative stress enhances the immune response to oxidatively modified catalase enzyme in patients with Graves' disease.
Gargouri, Bochra; Mseddi, Malek; Mnif, Fatma; Abid, Mohamed; Attia, Hamadi; Lassoued, Saloua.
Afiliação
  • Gargouri B; Faculty of Sciences of Sfax Tunisia, Sfax, Tunisia.
  • Mseddi M; Laboratory LR11ES45, Research Group "Biotechnology and Pathology", National School of Engineers of Sfax, Sfax, Tunisia.
  • Mnif F; Department of endocrinology, Hedi Chaker Hospital, Sfax, Tunisia.
  • Abid M; Department of endocrinology, Hedi Chaker Hospital, Sfax, Tunisia.
  • Attia H; Laboratory LR11ES45, Research Group "Biotechnology and Pathology", National School of Engineers of Sfax, Sfax, Tunisia.
  • Lassoued S; Laboratory LR11ES45, Research Group "Biotechnology and Pathology", National School of Engineers of Sfax, Sfax, Tunisia.
J Clin Lab Anal ; 34(2): e23051, 2020 Feb.
Article em En | MEDLINE | ID: mdl-31617239
ABSTRACT

BACKGROUND:

Oxidative stress is associated with several autoimmune disorders and oxidative modification of proteins that may result in autoimmune response. This study aims to evaluate the catalase (CAT) activity and the autoimmune response against the native CAT and the oxidatively modified enzyme in patients with Graves' disease (GD) and healthy controls in a comparative way.

METHODS:

The CAT activity was evaluated via spectrophotometric method. Using enzyme-linked immunosorbent assay, the reactivities of autoantibody toward native, malondialdehyde (MDA) and hydrogen peroxide (H2 O2 ) modified CAT were evaluated in plasmas of patients and controls.

RESULTS:

Reduced CAT activity was found in patients compared with controls (P < .05). It was proved that levels of IgG antibodies against MDA-modified CAT were higher than against unmodified ones (P < .001). No changes were found for the reactivities to H2 O2 -modified CAT. Positive correlation was found between the reactivity to MDA-modified CAT and the triiodothyronine level (P < .001, r = .6).

CONCLUSION:

Our findings incriminate the MDA in the autoantibodies reactivity to oxidatively modified CAT leading to a disturbed oxidative profile and/or the progression of GD pathology.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2020 Tipo de documento: Article