Your browser doesn't support javascript.
loading
Metabolomics of endemic six Astragalus species by combined NMR and GC-MS analysis.
Sahin Yaglioglu, Ayse; Temirturk, Murat; Ugur, Emic; Dolarslan, Melda; Demirtas, Ibrahim.
Afiliação
  • Sahin Yaglioglu A; Department of Chemistry, Cankiri Karatekin Universitesi, Cankiri, Turkey.
  • Temirturk M; Department of Chemistry, Cankiri Karatekin Universitesi, Cankiri, Turkey.
  • Ugur E; Department of Chemistry, Cankiri Karatekin Universitesi, Cankiri, Turkey.
  • Dolarslan M; School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, UK.
  • Demirtas I; Department of Chemistry, Cankiri Karatekin Universitesi, Cankiri, Turkey.
Phytochem Anal ; 31(3): 306-313, 2020 May.
Article em En | MEDLINE | ID: mdl-31943462
INTRODUCTION: Astragalus anthylloides, A. dipsaceus, A. karamasicus, A. lycius, A. sigmoideus and A. xylobasis var. angustus are an endemic and generally grow in the Irano-Turanian phytogeographic region of Turkey. Astragalus species contain saponins, polysaccharides, and phenolics, while the toxic compounds include imidazoline alkaloids, nitro toxins, and selenium derivatives. OBJECTIVES: To apply a combined metabolomic fingerprinting approach by nuclear magnetic resonance (NMR) and gas chromatography-mass spectrometry (GC-MS) of endemic six Astragalus species extract. METHODOLOGY: The whole plant collected in Turkey of six endemic Astragalus subsp. were dried and then extracted with hexane, chloroform, ethylacetate, n-butanol and methanol solvents, respectively. The hexane extracts were analyzed by GC-MS. Carbon-13 (13 C)-NMR analyzes of all extracts were performed. In both analyses, a biomarker was obtained. RESULTS: The hexane extracts were determined as palmitic acid, arachidic acid, behenic acid, and linolenic acid as the main components. As a result of 13 C-NMR analyzes, in hexane, chloroform, and ethylacetate the extracts detected were palmitic acid, arachidic acid, behenic acid, and linolenic acid. d-Pinitol was obtained using 13 C-NMR analyzes with n-butanol and methanol extracts. CONCLUSION: This study demonstrated that d-pinitol is a biomarker for the endemic six Astragalus subsp.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE País/Região como assunto: Asia Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE País/Região como assunto: Asia Idioma: En Ano de publicação: 2020 Tipo de documento: Article