Thermal shift assay: Strengths and weaknesses of the method to investigate the ligand-induced thermostabilization of soluble guanylyl cyclase.

Elgert, Christin; Rühle, Anne; Sandner, Peter; Behrends, Sönke

Elgert, Christin; Rühle, Anne; Sandner, Peter; Behrends, Sönke.

Afiliação

Elgert C; Department of Pharmacology, Toxicology and Clinical Pharmacy, University of Braunschweig - Institute of Technology, Germany. Electronic address: c.elgert@tu-braunschweig.de.
Rühle A; Department of Pharmacology, Toxicology and Clinical Pharmacy, University of Braunschweig - Institute of Technology, Germany. Electronic address: a.ruehle@tu-braunschweig.de.
Sandner P; Bayer AG, Drug Discovery, Wuppertal, Germany. Electronic address: peter.sandner@bayer.com.
Behrends S; Department of Pharmacology, Toxicology and Clinical Pharmacy, University of Braunschweig - Institute of Technology, Germany. Electronic address: s.behrends@tu-braunschweig.de.

J Pharm Biomed Anal ; 181: 113065, 2020 Mar 20.

Article em En | MEDLINE | ID: mdl-32032919

Assuntos

Análise Diferencial Térmica/métodos; Estabilidade Enzimática/efeitos dos fármacos; Guanilil Ciclase Solúvel/química; Benzoatos/farmacologia; Compostos de Bifenilo/farmacologia; Hidrocarbonetos Fluorados/farmacologia; Ligantes; Nucleotídeos/farmacologia; Temperatura de Transição

Palavras-chave

Guanylyl cyclase; Melting point; Nitric oxide; Protein thermal shift assay; Thermostability; sGC activators

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2020 Tipo de documento: Article