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Historic recombination in a durum wheat breeding panel enables high-resolution mapping of Fusarium head blight resistance quantitative trait loci.
Sari, Ehsan; Knox, Ron E; Ruan, Yuefeng; Henriquez, Maria Antonia; Kumar, Santosh; Burt, Andrew J; Cuthbert, Richard D; Konkin, David J; Walkowiak, Sean; Campbell, Heather L; Singh, Asheesh K; Ross, Jay; Lokuruge, Prabhath; Hsueh, Emma; Boyle, Kerry; Sidebottom, Christine; Condie, Janet; Yates, Shawn; Pozniak, Curtis J; Fobert, Pierre R.
Afiliação
  • Sari E; Aquatic and Crop Resource Development Centre, National Research Council, Saskatoon, SK, Canada.
  • Knox RE; Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada.
  • Ruan Y; Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada. ron.knox@canada.ca.
  • Henriquez MA; Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada. yuefeng.ruan@canada.ca.
  • Kumar S; Morden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, MB, Canada.
  • Burt AJ; Brandon Research and Development Centre, Agriculture and Agri-Food Canada, Brandon, MB, Canada.
  • Cuthbert RD; Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
  • Konkin DJ; Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada.
  • Walkowiak S; Aquatic and Crop Resource Development Centre, National Research Council, Saskatoon, SK, Canada.
  • Campbell HL; Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada.
  • Singh AK; Canadian Grain Commission, Winnipeg, MB, Canada.
  • Ross J; Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada.
  • Lokuruge P; Department of Agronomy, Iowa State University, Ames, Iowa, United States of America.
  • Hsueh E; Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada.
  • Boyle K; Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada.
  • Sidebottom C; Aquatic and Crop Resource Development Centre, National Research Council, Saskatoon, SK, Canada.
  • Condie J; Aquatic and Crop Resource Development Centre, National Research Council, Saskatoon, SK, Canada.
  • Yates S; Aquatic and Crop Resource Development Centre, National Research Council, Saskatoon, SK, Canada.
  • Pozniak CJ; Aquatic and Crop Resource Development Centre, National Research Council, Saskatoon, SK, Canada.
  • Fobert PR; Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada.
Sci Rep ; 10(1): 7567, 2020 05 05.
Article em En | MEDLINE | ID: mdl-32372012
The durum wheat line DT696 is a source of moderate Fusarium head blight (FHB) resistance. Previous analysis using a bi-parental population identified two FHB resistance quantitative trait loci (QTL) on chromosome 5A: 5A1 was co-located with a plant height QTL, and 5A2 with a major maturity QTL. A Genome-Wide Association Study (GWAS) of DT696 derivative lines from 72 crosses based on multi-environment FHB resistance, plant height, and maturity phenotypic data was conducted to improve the mapping resolution and further elucidate the genetic relationship of height and maturity with FHB resistance. The Global Tetraploid Wheat Collection (GTWC) was exploited to identify durum wheat lines with DT696 allele and additional recombination events. The 5A2 QTL was confirmed in the derivatives, suggesting the expression stability of the 5A2 QTL in various genetic backgrounds. The GWAS led to an improved mapping resolution rendering the 5A2 interval 10 Mbp shorter than the bi-parental QTL mapping interval. Haplotype analysis using SNPs within the 5A2 QTL applied to the GTWC identified novel haplotypes and recombination breakpoints, which could be exploited for further improvement of the mapping resolution. This study suggested that GWAS of derivative breeding lines is a credible strategy for improving mapping resolution.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Article