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Mechanism of Polymer-Mediated Cryopreservation Using Poly(methyl glycidyl sulfoxide).
Burkey, Aaron A; Hillsley, Alexander; Harris, Dale T; Baltzegar, Jacob R; Zhang, Diana Y; Sprague, William W; Rosales, Adrianne M; Lynd, Nathaniel A.
Afiliação
  • Burkey AA; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas, United States.
  • Hillsley A; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas, United States.
  • Harris DT; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas, United States.
  • Baltzegar JR; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas, United States.
  • Zhang DY; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas, United States.
  • Sprague WW; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas, United States.
  • Rosales AM; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas, United States.
  • Lynd NA; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas, United States.
Biomacromolecules ; 21(8): 3047-3055, 2020 08 10.
Article em En | MEDLINE | ID: mdl-32649830
Under the right conditions, some biological systems can maintain high viability after being frozen and thawed, but many others (e.g., organs and many mammalian cells) cannot. To increase the rates of post-thaw viability and widen the library of living cells and tissues that can be stored frozen, an improved understanding of the mode of action of polymeric cryoprotectants is required. Here, we present a polymeric cryoprotectant, poly(methyl glycidyl sulfoxide) (PMGS), that achieved higher post-thaw viability for fibroblast cells than its small-molecule analogue dimethyl sulfoxide. By limiting the amount of water that freezes and facilitating cellular dehydration after ice nucleation, PMGS mitigates the mechanical and osmotic stresses that the freezing of water imparts on cells and facilitates higher-temperature vitrification of the remaining unfrozen volume. The development of PMGS advances a fundamental physical understanding of polymer-mediated cryopreservation, which enables new material design for long-term preservation of complex cellular networks and tissue.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article