Analysis of metabolically labeled inositol phosphate messengers by NMR.
Methods Enzymol
; 641: 35-52, 2020.
Article
em En
| MEDLINE
| ID: mdl-32713530
ABSTRACT
Inositol phosphates (InsPs) are an important group of eukaryotic messengers and mediate a wide range of processes. To elucidate the biological functions of these molecules, robust techniques to characterize inositol phosphate metabolism at the cellular level are highly sought after. This chapter provides a detailed protocol for the preparation of 13C-labeled myo-inositol, its use for metabolic labeling of mammalian and yeast cells, and the quantitative analysis of intracellular InsP pools from cell extracts using NMR spectroscopy.
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01-internacional
Base de dados:
MEDLINE
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article