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Thermal and pH Stabilities of i-DNA: Confronting in vitro Experiments with Models and In-Cell NMR Data.
Cheng, Mingpan; Qiu, Dehui; Tamon, Liezel; Istvánková, Eva; Vísková, Pavlína; Amrane, Samir; Guédin, Aurore; Chen, Jielin; Lacroix, Laurent; Ju, Huangxian; Trantírek, Lukás; Sahakyan, Aleksandr B; Zhou, Jun; Mergny, Jean-Louis.
Afiliação
  • Cheng M; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing, 210023, China.
  • Qiu D; ARNA Laboratory, Université de Bordeaux, INSERM U 1212, CNRS UMR5320, IECB, 33607, Pessac, France.
  • Tamon L; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing, 210023, China.
  • Istvánková E; MRC WIMM Centre for Computational Biology, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, OX3 9DS, UK.
  • Vísková P; Central European Institute of Technology, Masaryk University, 62500, Brno, Czech Republic.
  • Amrane S; Central European Institute of Technology, Masaryk University, 62500, Brno, Czech Republic.
  • Guédin A; ARNA Laboratory, Université de Bordeaux, INSERM U 1212, CNRS UMR5320, IECB, 33607, Pessac, France.
  • Chen J; ARNA Laboratory, Université de Bordeaux, INSERM U 1212, CNRS UMR5320, IECB, 33607, Pessac, France.
  • Lacroix L; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing, 210023, China.
  • Ju H; IBENS, Ecole Normale Supérieure, CNRS, INSERM, PSL Research University, 75005, Paris, France.
  • Trantírek L; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing, 210023, China.
  • Sahakyan AB; Central European Institute of Technology, Masaryk University, 62500, Brno, Czech Republic.
  • Zhou J; MRC WIMM Centre for Computational Biology, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, OX3 9DS, UK.
  • Mergny JL; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing, 210023, China.
Angew Chem Int Ed Engl ; 60(18): 10286-10294, 2021 04 26.
Article em En | MEDLINE | ID: mdl-33605024
Recent studies indicate that i-DNA, a four-stranded cytosine-rich DNA also known as the i-motif, is actually formed in vivo; however, a systematic study on sequence effects on stability has been missing. Herein, an unprecedented number of different sequences (271) bearing four runs of 3-6 cytosines with different spacer lengths has been tested. While i-DNA stability is nearly independent on total spacer length, the central spacer plays a special role on stability. Stability also depends on the length of the C-tracts at both acidic and neutral pHs. This study provides a global picture on i-DNA stability thanks to the large size of the introduced data set; it reveals unexpected features and allows to conclude that determinants of i-DNA stability do not mirror those of G-quadruplexes. Our results illustrate the structural roles of loops and C-tracts on i-DNA stability, confirm its formation in cells, and allow establishing rules to predict its stability.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article