A Simple Method for In-Depth Proteome Analysis of Mammalian Cell Culture Conditioned Media Containing Fetal Bovine Serum.

Nakamura, Ren; Nakajima, Daisuke; Sato, Hironori; Endo, Yusuke; Ohara, Osamu; Kawashima, Yusuke

Nakamura, Ren; Nakajima, Daisuke; Sato, Hironori; Endo, Yusuke; Ohara, Osamu; Kawashima, Yusuke.

Afiliação

Nakamura R; Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
Nakajima D; Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
Sato H; Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
Endo Y; Department of Frontier Research and Development, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
Ohara O; Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
Kawashima Y; Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.

Int J Mol Sci ; 22(5)2021 Mar 04.

Article em En | MEDLINE | ID: mdl-33806404

Assuntos

Técnicas de Cultura de Células/métodos; Meios de Cultivo Condicionados/química; Proteoma/análise; Animais; Bovinos; Cromatografia Líquida; Células HeLa; Humanos; Proteômica/métodos; Soroalbumina Bovina/química; Soroalbumina Bovina/isolamento & purificação; Espectrometria de Massas em Tandem; Fator de Necrose Tumoral alfa/farmacologia

Palavras-chave

DIA; albumin removal; conditioned medium; fetal bovine serum; proteome analysis; secreted proteins

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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