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LGR6 marks nephron progenitor cells.
van Ineveld, Ravian L; Margaritis, Thanasis; Kooiman, Berend A P; Groenveld, Femke; Ariese, Hendrikus C R; Lijnzaad, Philip; Johnson, Hannah R; Korving, Jeroen; Wehrens, Ellen J; Holstege, Frank; van Rheenen, Jacco; Drost, Jarno; Rios, Anne C; Bos, Frank L.
Afiliação
  • van Ineveld RL; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
  • Margaritis T; Oncode Institute, Utrecht, The Netherlands.
  • Kooiman BAP; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
  • Groenveld F; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
  • Ariese HCR; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht, Utrecht, The Netherlands.
  • Lijnzaad P; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
  • Johnson HR; Oncode Institute, Utrecht, The Netherlands.
  • Korving J; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
  • Wehrens EJ; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
  • Holstege F; Oncode Institute, Utrecht, The Netherlands.
  • van Rheenen J; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht, Utrecht, The Netherlands.
  • Drost J; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
  • Rios AC; Oncode Institute, Utrecht, The Netherlands.
  • Bos FL; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
Dev Dyn ; 250(11): 1568-1583, 2021 11.
Article em En | MEDLINE | ID: mdl-33848015
BACKGROUND: Nephron progenitor cells (NPCs) undergo a stepwise process to generate all mature nephron structures. Mesenchymal to epithelial transition (MET) is considered a multistep process of NPC differentiation to ensure progressive establishment of new nephrons. However, despite this important role, to date, no marker for NPCs undergoing MET in the nephron exists. RESULTS: Here, we identify LGR6 as a NPC marker, expressed in very early cap mesenchyme, pre-tubular aggregates, renal vesicles, and in segments of S-shaped bodies, following the trajectory of MET. By using a lineage tracing approach in embryonic explants in combination with confocal imaging and single-cell RNA sequencing, we provide evidence for the multiple fates of LGR6+ cells during embryonic nephrogenesis. Moreover, by using long-term in vivo lineage tracing, we show that postnatal LGR6+ cells are capable of generating the multiple lineages of the nephrons. CONCLUSIONS: Given the profound early mesenchymal expression and MET signature of LGR6+ cells, together with the lineage tracing of mesenchymal LGR6+ cells, we conclude that LGR6+ cells contribute to all nephrogenic segments by undergoing MET. LGR6+ cells can therefore be considered an early committed NPC population during embryonic and postnatal nephrogenesis with potential regenerative capability.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article