Optimizing SARS-CoV-2 Surveillance in the United States: Insights From the National Football League Occupational Health Program.
Ann Intern Med
; 174(8): 1081-1089, 2021 08.
Article
em En
| MEDLINE
| ID: mdl-34125571
ABSTRACT
BACKGROUND:
Evidence to understand effective strategies for surveillance and early detection of SARS-CoV-2 is limited.OBJECTIVE:
To describe the results of a rigorous, large-scale COVID-19 testing and monitoring program.DESIGN:
The U.S. National Football League (NFL) and the NFL Players Association (NFLPA) instituted a large-scale COVID-19 monitoring program involving daily testing using 2 reverse transcription polymerase chain reaction (RT-PCR) platforms (Roche cobas and Thermo Fisher QuantStudio), a transcription-mediated amplification platform (Hologic Panther), and an antigen point-of-care (aPOC) test (Quidel Sofia).SETTING:
32 NFL clubs in 24 states during the 2020 NFL season.PARTICIPANTS:
NFL players and staff. MEASUREMENTS SARS-CoV-2 test results were described in the context of medically adjudicated status. Cycle threshold (Ct) values are reported when available.RESULTS:
A total of 632 370 tests administered across 11 668 persons identified 270 (2.4%) COVID-19 cases from 1 August to 14 November 2020. Positive predictive values ranged from 73.0% to 82.0% across the RT-PCR platforms. High Ct values (33 to 37) often indicated early infection. For the first positive result, the median Ct value was 32.77 (interquartile range, 30.02 to 34.72) and 22% of Ct values were above 35. Among adjudicated COVID-19 cases tested with aPOC, 42.3% had a negative result. Positive concordance between aPOC test result and adjudicated case status increased as viral load increased.LIMITATIONS:
Platforms varied by laboratory, and test variability may reflect procedural differences.CONCLUSION:
Routine RT-PCR testing allowed early detection of infection. Cycle threshold values provided a useful guidepost for understanding results, with high values often indicating early infection. Antigen POC testing was unable to reliably rule out COVID-19 early in infection. PRIMARY FUNDING SOURCE The NFL and the NFLPA.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
/
Incidence_studies
/
Prognostic_studies
/
Screening_studies
Limite:
Humans
País/Região como assunto:
America do norte
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article