Dielectric Spectroscopy Can Predict the Effect of External AC Fields on the Dynamic Adsorption of Lysozyme.
Chemphyschem
; 23(10): e202100914, 2022 05 18.
Article
em En
| MEDLINE
| ID: mdl-35226788
ABSTRACT
This report describes the application of dielectric spectroscopy as a simple and fast way to guide protein adsorption experiments. Specifically, the polarization behavior of a layer of adsorbed lysozyme was investigated using a triangular-wave signal with frequencies varying from 0.5 to 2â
Hz. The basic experiment, which can be performed in less than 5â
min and with a single sample, not only allowed confirming the susceptibility of the selected protein towards the electric signal but also identified that this protein would respond more efficiently to signals with lower frequencies. To verify the validity of these observations, the adsorption behavior of lysozyme onto optically transparent carbon electrodes was also investigated under the influence of an applied alternating potential. In these experiments, the applied signal was defined by a sinusoidal wave with an amplitude of 100â
mV and superimposed to +800â
mV (applied as a working potential) and varying the frequency in the 0.1-10000â
Hz range. The experimental data showed that the greatest adsorbed amounts of lysozyme were obtained at the lowest tested frequencies (0.1-1.0â
Hz), results that are in line with the corresponding dielectric features of the protein.
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01-internacional
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MEDLINE
Tipo de estudo:
Prognostic_studies
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Risk_factors_studies
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article