Testing the polar auxin transport model with a selective plasma membrane H+ -ATPase inhibitor.
J Integr Plant Biol
; 64(6): 1229-1245, 2022 Jun.
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| ID: mdl-35352470
ABSTRACT
Auxin is unique among plant hormones in that its function requires polarized transport across plant cells. A chemiosmotic model was proposed to explain how polar auxin transport is derived by the H+ gradient across the plasma membrane (PM) established by PM H+ -adenosine triphosphatases (ATPases). However, a classical genetic approach by mutations in PM H+ -ATPase members did not result in the ablation of polar auxin distribution, possibly due to functional redundancy in this gene family. To confirm the crucial role of PM H+ -ATPases in the polar auxin transport model, we employed a chemical genetic approach. Through a chemical screen, we identified protonstatin-1 (PS-1), a selective small-molecule inhibitor of PM H+ -ATPase activity that inhibits auxin transport. Assays with transgenic plants and yeast strains showed that the activity of PM H+ -ATPases affects auxin uptake as well as acropetal and basipetal polar auxin transport. We propose that PS-1 can be used as a tool to interrogate the function of PM H+ -ATPases. Our results support the chemiosmotic model in which PM H+ -ATPase itself plays a fundamental role in polar auxin transport.
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MEDLINE
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En
Ano de publicação:
2022
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Article