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miR-150-5p and let-7b-5p in Blood Myeloid Extracellular Vesicles Track Cognitive Symptoms in Patients with Multiple Sclerosis.
Scaroni, Federica; Visconte, Caterina; Serpente, Maria; Golia, Maria Teresa; Gabrielli, Martina; Huiskamp, Marijn; Hulst, Hanneke E; Carandini, Tiziana; De Riz, Milena; Pietroboni, Anna; Rotondo, Emanuela; Scarpini, Elio; Galimberti, Daniela; Teunissen, Charlotte E; van Dam, Maureen; de Jong, Brigit A; Fenoglio, Chiara; Verderio, Claudia.
Afiliação
  • Scaroni F; Institute of Neuroscience, CNR, Via Follereau 3, 20854 Vedano al Lambro, Italy.
  • Visconte C; Department of Biomedical, Surgical and Dental Sciences, University of Milan, Via F. Sforza 35, 20122 Milan, Italy.
  • Serpente M; Centro Dino Ferrari, University of Milan, 20122 Milan, Italy.
  • Golia MT; Centro Dino Ferrari, University of Milan, 20122 Milan, Italy.
  • Gabrielli M; Fondazione IRCCS Ca' Granda, Ospedale Policlinico, 20122 Milan, Italy.
  • Huiskamp M; Institute of Neuroscience, CNR, Via Follereau 3, 20854 Vedano al Lambro, Italy.
  • Hulst HE; Institute of Neuroscience, CNR, Via Follereau 3, 20854 Vedano al Lambro, Italy.
  • Carandini T; MS Center Amsterdam, Amsterdam Neuroscience, Department of Anatomy and Neurosciences, Amsterdam UMC, Vrije Universiteit Amsterdam UMC, De Boelelaan 1117, 1081 Amsterdam, The Netherlands.
  • De Riz M; Health-, Medical- and Neuropsychology Unit, Institute of Psychology, Leiden University, 2300 Leiden, The Netherlands.
  • Pietroboni A; Centro Dino Ferrari, University of Milan, 20122 Milan, Italy.
  • Rotondo E; Fondazione IRCCS Ca' Granda, Ospedale Policlinico, 20122 Milan, Italy.
  • Scarpini E; Centro Dino Ferrari, University of Milan, 20122 Milan, Italy.
  • Galimberti D; Fondazione IRCCS Ca' Granda, Ospedale Policlinico, 20122 Milan, Italy.
  • Teunissen CE; Centro Dino Ferrari, University of Milan, 20122 Milan, Italy.
  • van Dam M; Fondazione IRCCS Ca' Granda, Ospedale Policlinico, 20122 Milan, Italy.
  • de Jong BA; Centro Dino Ferrari, University of Milan, 20122 Milan, Italy.
  • Fenoglio C; Fondazione IRCCS Ca' Granda, Ospedale Policlinico, 20122 Milan, Italy.
  • Verderio C; Department of Biomedical, Surgical and Dental Sciences, University of Milan, Via F. Sforza 35, 20122 Milan, Italy.
Cells ; 11(9)2022 05 05.
Article em En | MEDLINE | ID: mdl-35563859
Cognitive deficits strongly affect the quality of life of patients with multiple sclerosis (MS). However, no cognitive MS biomarkers are currently available. Extracellular vesicles (EVs) contain markers of parental cells and are able to pass from the brain into blood, representing a source of disease biomarkers. The aim of this study was to investigate whether small non-coding microRNAs (miRNAs) targeting synaptic genes and packaged in plasma EVs may reflect cognitive deficits in MS patients. Total EVs were precipitated by Exoquick from the plasma of twenty-six cognitively preserved (CP) and twenty-three cognitively impaired (CI) MS patients belonging to two independent cohorts. Myeloid EVs were extracted by affinity capture from total EVs using Isolectin B4 (IB4). Fourteen miRNAs targeting synaptic genes were selected and measured by RT-PCR in both total and myeloid EVs. Myeloid EVs from CI patients expressed higher levels of miR-150-5p and lower levels of let-7b-5p compared to CP patients. Stratification for progressive MS (PMS) and relapsing-remitting MS (RRMS) and correlation with clinical parameters suggested that these alterations might be attributable to cognitive deficits rather than disease progression. This study identifies miR-150-5p and let-7b-5p packaged in blood myeloid EVs as possible biomarkers for cognitive deficits in MS.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article