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Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro.
Hromada, Carina; Hartmann, Jaana; Oesterreicher, Johannes; Stoiber, Anton; Daerr, Anna; Schädl, Barbara; Priglinger, Eleni; Teuschl-Woller, Andreas H; Holnthoner, Wolfgang; Heinzel, Johannes; Hercher, David.
Afiliação
  • Hromada C; Department Life Science Engineering, University of Applied Sciences Technikum Wien, 1200 Vienna, Austria.
  • Hartmann J; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria.
  • Oesterreicher J; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria.
  • Stoiber A; Ludwig Boltzmann Institute for Traumatology, The Research Centre in Cooperation with AUVA, 1200 Vienna, Austria.
  • Daerr A; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria.
  • Schädl B; Ludwig Boltzmann Institute for Traumatology, The Research Centre in Cooperation with AUVA, 1200 Vienna, Austria.
  • Priglinger E; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria.
  • Teuschl-Woller AH; Ludwig Boltzmann Institute for Traumatology, The Research Centre in Cooperation with AUVA, 1200 Vienna, Austria.
  • Holnthoner W; Department Life Science Engineering, University of Applied Sciences Technikum Wien, 1200 Vienna, Austria.
  • Heinzel J; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria.
  • Hercher D; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria.
Biomolecules ; 12(6)2022 06 12.
Article em En | MEDLINE | ID: mdl-35740945
Peripheral nerve injuries pose a major clinical concern world-wide, and functional recovery after segmental peripheral nerve injury is often unsatisfactory, even in cases of autografting. Although it is well established that angiogenesis plays a pivotal role during nerve regeneration, the influence of lymphangiogenesis is strongly under-investigated. In this study, we analyzed the presence of lymphatic vasculature in healthy and regenerated murine peripheral nerves, revealing that nerve autografts contained increased numbers of lymphatic vessels after segmental damage. This led us to elucidate the interaction between lymphatic endothelial cells (LECs) and Schwann cells (SCs) in vitro. We show that SC and LEC secretomes did not influence the respective other cell types' migration and proliferation in 2D scratch assay experiments. Furthermore, we successfully created lymphatic microvascular structures in SC-embedded 3D fibrin hydrogels, in the presence of supporting cells; whereas SCs seemed to exert anti-lymphangiogenic effects when cultured with LECs alone. Here, we describe, for the first time, increased lymphangiogenesis after peripheral nerve injury and repair. Furthermore, our findings indicate a potential lymph-repellent property of SCs, thereby providing a possible explanation for the lack of lymphatic vessels in the healthy endoneurium. Our results highlight the importance of elucidating the molecular mechanisms of SC-LEC interaction.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article