Direct capture and amplification of nucleic acids using a universal, elution-free magnetic bead-based method for rapid pathogen detection in multiple types of biological samples.

Jiang, Qianqian; Li, Yang; Huang, Lin; Guo, Jinling; Wang, Ailin; Ma, Cuiping; Shi, Chao

Jiang, Qianqian; Li, Yang; Huang, Lin; Guo, Jinling; Wang, Ailin; Ma, Cuiping; Shi, Chao.

Afiliação

Jiang Q; Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences; Department of Pathogenic Biology, School of Basic Medicine; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, Peopl
Li Y; Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences; Department of Pathogenic Biology, School of Basic Medicine; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, Peopl
Huang L; Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences; Department of Pathogenic Biology, School of Basic Medicine; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, Peopl
Guo J; Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences; Department of Pathogenic Biology, School of Basic Medicine; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, Peopl
Wang A; Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences; Department of Pathogenic Biology, School of Basic Medicine; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, Peopl
Ma C; Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People's Republic of China.
Shi C; Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences; Department of Pathogenic Biology, School of Basic Medicine; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, Peopl

Anal Bioanal Chem ; 415(3): 427-438, 2023 Jan.

Article em En | MEDLINE | ID: mdl-36385304

Assuntos

Ácidos Nucleicos; Técnicas de Amplificação de Ácido Nucleico/métodos; Reação em Cadeia da Polimerase em Tempo Real/métodos; Fenômenos Magnéticos; Sensibilidade e Especificidade

Palavras-chave

Elution-free; Magnetic beads; Nucleic acid amplification tests; Nucleic acid extraction; Pathogen detection; Polyethylene-polypropylene glycol F68

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article