[Effects of octadecadienoic acid on proliferation and apoptosis of glioma cells and its mechanisms].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
; 38(5): 434-437, 2022 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-37088747
ABSTRACT
OBJECTIVE:
To study the effects of octadecadienoic acid (ODA) on the proliferation and apoptosis of glioma cells and its mechanisms.METHODS:
Cultured human glioma cells (cell density 2×106 cells/L) were divided into solvent control group (DMSO, 30 µl/L), 5-FU group (10 mg/L) and octadecadienic acid groups (0.3, 0.6 and 1.2 mg/L groups). The toxicity of ODA on glioma cells was detected by trypan blue and thiazolium blue (MTT). The expression levels of P53, PI3K, P21, PKB/Akt and Caspase-9 in glioma cells were determined by enzyme-linked immunosorbent assay (ELISA).RESULTS:
â Cell count under optical microscope showed that the inhibition rate of cell proliferation in ODA low, medium and high dose groups and 5-FU group was significantly higher than that in the solvent control group (Pï¼0.01), but there was no statistical significance compared with the 5-FU group (Pï¼0.05). â¡ MTT assay showed that the inhibition rate of cell proliferation was increased significantly in ODA low, medium and high dose groups and 5-FU groups (Pï¼0.01), compared with the solvent control group. Compared with 5-FU group, the inhibition rate of cell proliferation was increased significantly only in ODA high dose group (Pï¼0.01). ⢠The number of G0/G1 phase cells in ODA low, medium and high dose groups and 5-FU group were increased significantly (Pï¼0.05, Pï¼0.01), the number of G2/M phase cells were decreased significantly (Pï¼0.01), and the apoptosis rate was increased significantly (Pï¼0.01),compared with the solvent control group. Compared with the 5-FU group, the number of cells in G2/M phase was decreased significantly (Pï¼0.01) and the apoptosis rate was increased significantly (Pï¼0.01) in ODA high dose group. ⣠ELISA test results showed that the protein expression levels of P53, PI3K and PKB/Akt in ODA low , medium and high dose groups and 5-FU group were significantly lower than those in solvent control group (all Pï¼0.01), but the protein expression levels in ODA high dose group were significantly lower than those in 5-FU group (Pï¼0.01). The protein expression levels of P21 and caspase-9 in ODA low , medium and high dose groups and 5-FU group were significantly higher than those in solvent control group (Pï¼0.05, Pï¼0.01), but the protein expression levels in ODA high dose group were significantly higher than those in 5-Fu group (Pï¼0.01).CONCLUSION:
ODA can significantly inhibit the proliferation and promote apoptosis of glioma cells. The mechanisms are related to up-regulating the levels of P21 and caspase-9 to promote apoptosis, down-regulating the levels of P53, PI3K and PKB/Akt to inhibit the cell division cycle, and reducing the activity of PI3K-Akt signal transduction pathway.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2022
Tipo de documento:
Article