A recombinant technique for mapping functional sites of heterotrimeric collagen helices: Collagen IV CB3 fragment as a prototype for integrin binding.

Boudko, Sergei P; Konopka, Elizabeth H; Kim, Woojin; Taga, Yuki; Mizuno, Kazunori; Springer, Timothy A; Hudson, Billy G; Moy, Terence I; Lin, Fu-Yang

Boudko, Sergei P; Konopka, Elizabeth H; Kim, Woojin; Taga, Yuki; Mizuno, Kazunori; Springer, Timothy A; Hudson, Billy G; Moy, Terence I; Lin, Fu-Yang.

Afiliação

Boudko SP; Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA; Center for Matrix Biology, Vanderbilt University Medical Center, Nashville, Tennessee, USA; Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, USA.
Konopka EH; Morphic Therapeutic, Inc, Waltham, Massachusetts, USA.
Kim W; Morphic Therapeutic, Inc, Waltham, Massachusetts, USA.
Taga Y; Nippi Research Institute of Biomatrix, Toride, Ibaraki, Japan.
Mizuno K; Nippi Research Institute of Biomatrix, Toride, Ibaraki, Japan.
Springer TA; Department of Biological Chemistry and Molecular Pharmacology, Program in Cellular and Molecular Medicine, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Hudson BG; Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA; Center for Matrix Biology, Vanderbilt University Medical Center, Nashville, Tennessee, USA; Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, USA;
Moy TI; Morphic Therapeutic, Inc, Waltham, Massachusetts, USA.
Lin FY; Morphic Therapeutic, Inc, Waltham, Massachusetts, USA. Electronic address: albert.lin@morphictx.com.

J Biol Chem ; 299(7): 104901, 2023 07.

Article em En | MEDLINE | ID: mdl-37302550

Assuntos

Colágeno Tipo IV; Integrinas; Multimerização Proteica; Animais; Humanos; Sítios de Ligação; Colágeno Tipo IV/química; Colágeno Tipo IV/genética; Colágeno Tipo IV/metabolismo; Integrinas/química; Integrinas/metabolismo; Ligação Proteica; Estrutura Secundária de Proteína; Mutação; Domínios Proteicos

Palavras-chave

CB3 fragment; CD spectroscopy; atomic force microscopy; basement membrane; collagen IV; cystine knot; extracellular matrix; heterotrimer; integrin; protein folding; protein self-assembly; receptor; recombinant protein expression; triple helix

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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PubMed Links

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article