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Chromosome-level reference genomes of two imperiled desert fishes: spikedace (Meda fulgida) and loach minnow (Tiaroga cobitis).
Alexandre, Nicolas M; Cameron, Alexander C; Tian, David; Chatla, Kamalakar; Kolora, Sree R R; Whiteman, Noah K; Turner, Thomas F; Reinthal, Peter N.
Afiliação
  • Alexandre NM; Department of Integrative Biology, University of California, Berkeley, CA 94720, USA.
  • Cameron AC; Museum of Vertebrate Zoology, Berkeley, CA 94720, USA.
  • Tian D; Museum of Southwestern Biology and Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA.
  • Chatla K; Department of Integrative Biology, University of California, Berkeley, CA 94720, USA.
  • Kolora SRR; Museum of Vertebrate Zoology, Berkeley, CA 94720, USA.
  • Whiteman NK; Department of Integrative Biology, University of California, Berkeley, CA 94720, USA.
  • Turner TF; Museum of Vertebrate Zoology, Berkeley, CA 94720, USA.
  • Reinthal PN; Department of Integrative Biology, University of California, Berkeley, CA 94720, USA.
G3 (Bethesda) ; 13(10)2023 09 30.
Article em En | MEDLINE | ID: mdl-37466215
ABSTRACT
North American minnows (Cypriniformes Leuciscidae) comprise a diverse taxonomic group, but many members, particularly those inhabiting deserts, face elevated extinction risks. Despite conservation concerns, leuciscids remain under sampled for reference assemblies relative to other groups of freshwater fishes. Here, we present 2 chromosome-scale reference genome assemblies spikedace (Meda fulgida) and loach minnow (Tiaroga cobitis) using PacBio, Illumina and Omni-C technologies. The complete assembly for spikedace was 882.1 Mb in total length comprised of 83 scaffolds with N50 = 34.8 Mb, L50 = 11, N75 = 32.3 Mb, and L75 = 18. The complete assembly for loach minnow was 1.3 Gb in total length comprised of 550 scaffolds with N50 = 48.6 Mb, L50 = 13, N75 = 42.3 Mb, and L75 = 20. Completeness assessed via Benchmarking Universal Single-Copy Orthologues (BUSCO) metrics using the Actinopterygii BUSCO database showed ∼97% for spikedace and ∼98% for loach minnow of complete BUSCO proportions. Annotation revealed approximately 32.58 and 29.04% of spikedace and loach minnow total genome lengths to be comprised of protein-coding genes, respectively. Comparative genomic analyses of these endangered and co-distributed fishes revealed widespread structural variants, gene family expansions, and evidence of positive selection in both genomes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article