Recombinant protein production for structural and kinetic studies: A case study using M. tuberculosis &#945;-methylacyl-CoA racemase (MCR).

Mojanaga, Otsile O; Acharya, K Ravi; Lloyd, Matthew D

Recombinant protein production for structural and kinetic studies: A case study using M. tuberculosis α-methylacyl-CoA racemase (MCR).

Mojanaga, Otsile O; Acharya, K Ravi; Lloyd, Matthew D.

Afiliação

Mojanaga OO; Department of Life Sciences, University of Bath, Claverton Down, Bath, United Kingdom.
Acharya KR; Department of Life Sciences, University of Bath, Claverton Down, Bath, United Kingdom. Electronic address: K.R.Acharya@bath.ac.uk.
Lloyd MD; Department of Life Sciences, University of Bath, Claverton Down, Bath, United Kingdom. Electronic address: M.D.Lloyd@bath.ac.uk.

Methods Enzymol ; 690: 1-37, 2023.

Article em En | MEDLINE | ID: mdl-37858526

Assuntos

Mycobacterium tuberculosis; Tuberculose; Humanos; Mycobacterium tuberculosis/genética; Mycobacterium tuberculosis/metabolismo; Cinética; Escherichia coli/genética; Escherichia coli/metabolismo; Sefarose/metabolismo; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Cromatografia de Afinidade/métodos

Palavras-chave

Anion-exchange chromatography; Cation-exchange chromatography; Expression and purification of recombinant proteins; Recombinant E. coli; SDS-PAGE; Size-exclusion chromatography; α-Methylacyl-CoA racemase

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article