In Vivo Wound Healing Model for Characterization of Gene Electrotransfer Effects in Mouse Skin.

ABSTRACT

Wound healing is a complex biological response to injury characterized by a sequence of interdependent and overlapping physiological actions. To study wound healing and cutaneous regeneration processes, the complexity of wound healing requires the use of animal models. In this chapter, we describe the protocol to generate skin wounds in a mouse model. In the mouse splinted excisional wound model, two full-thickness wounds are firstly created on the mouse dorsum, which is followed by application of silicone splint around wounded area. A splinting ring tightly adheres to the skin around full-thickness wound, preventing wound contraction and replicating human processes of re-epithelialization and new tissue formation. The wound is easily accessible for treatment as well as for daily monitoring and quantifying the wound closure.This technique represents valuable approach for the study of wound healing mechanisms and for evaluation of new therapeutic modalities. In this protocol, we describe how to utilize the model to study the effect of gene electrotransfer of plasmid DNA coding for antiangiogenic molecules. Additionally, we also present how to precisely regulate electrical parameters and modify electrode composition to reach optimal therapeutic effectiveness of gene electrotransfer into skin around wounded area.