Structural Characterization and In Vitro Anti-Inflammatory Activity of Polysaccharides Isolated from the Fruits of Rosa laevigata.
Int J Mol Sci
; 25(4)2024 Feb 09.
Article
em En
| MEDLINE
| ID: mdl-38396810
ABSTRACT
RLPa-2 (Mw 15.6 kDa) is a polysaccharide isolated from Rosa laevigata Michx. It consists of arabinose (Ara), galactose (Gal), rhamnose (Rha), glucose (Glc), xylose (Xyl), and galacturonic acid (Gal-UA) with a molar ratio of 1.000.910.390.340.250.20. Structural characterization was performed by methylation and NMR analysis, which indicated that RLPa-2 might comprise â6)-α-D-Galp-(1â, â4)-α-D-GalpA-(1â, α-L-Araf-(1â, â2,4)-α-D-Glcp-(1â, ß-D-Xylp, and α-L-Rhap. In addition, the bioactivity of RLPa-2 was assessed through an in vitro macrophage polarization assay. Compared to positive controls, there was a significant decrease in the expression of M1 macrophage markers (CD80, CD86) and p-STAT3/STAT3 protein. Additionally, there was a down-regulation in the production of pro-inflammatory mediators (NO, IL-6, TNF-α), indicating that M1 macrophage polarization induced with lipopolysaccharide (LPS) and interferon-γ (IFN-γ) stimulation could be inhibited by RLPa-2. These findings demonstrate that the RLPa-2 might be considered as a potential anti-inflammatory drug to reduce inflammation.
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MEDLINE
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En
Ano de publicação:
2024
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Article