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Using An In Vitro Tissue Perfusion System to Detect the Functional Activities of Isolated Intestinal Tubes in Real Time.
Guo, Pengmei; An, Wenqiao; Wang, Yili; Ren, Yajing; Zhang, Sanyin.
Afiliação
  • Guo P; Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine.
  • An W; School of Basic Medical College, Chengdu University of Traditional Chinese Medicine.
  • Wang Y; Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine.
  • Ren Y; School of Medical and Life Sciences, Chengdu University of Traditional Chinese Medicine.
  • Zhang S; Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine; Chengdu Integrated TCM and Western Medicine Hospital; tcmzsy@cdutcm.edu.cn.
J Vis Exp ; (209)2024 Jul 26.
Article em En | MEDLINE | ID: mdl-39141536
ABSTRACT
Gastrointestinal diseases, which have a high incidence, pose considerable challenges for humans. The small intestine is integral to food and drug digestion and absorption and plays a crucial role in treating these diseases. The intestinal tube movement experiment, a common and essential in vitro method, is utilized to study gastrointestinal dynamics. This includes the preparation of the isolated intestinal tube, as well as the suspension of the prepared intestinal tube in the bath and its connection to a signal detector. This is followed by the recording and analysis of a series of parameters, such as tension, which can be used to assess intestinal motor function, as well as considerations for keeping the intestinal tube active in vitro. The standardized program from sampling to data collection greatly improves the repeatability of the experimental data and ensures the authenticity of the recording of intestinal tension after physiological, pathological, and drug intervention. Here we present the key problems in experimental operation and a valuable reference experimental protocol for studying drugs that regulate gastrointestinal motility.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article