Insulin modulation of newly synthesized apolipoproteins B-100 and B-48 in human fetal intestine: gene expression and mRNA editing are not involved.
FEBS Lett
; 393(2-3): 253-8, 1996 Sep 16.
Article
em En
| MEDLINE
| ID: mdl-8814300
ABSTRACT
We investigated insulin's effect on intestinal lipid, transport and, particularly, the biogenesis of apolipoproteins crucial to lipoprotein secretion. Adding insulin (3 mU) to the serum-free medium of cultured jejunal explants from human fetuses (17-20 weeks) reduced triglyceride and chylomicron production and inhibited apo B-48 and apo B-100 secretion. When apo B mRNA was assayed by RT-PCR and its editing by primer extension, no change was detectable following the addition of insulin. HDL lipid content, apo A-1 synthesis and RNA level were unaffected by insulin. Collectively, these results suggest that the insulin-stimulated decline in intestinal chylomicron output may involve apo B co- or post-translational modifications.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article