The effects of Porphyromonas gingivalis infection on mononuclear cell adhesion to vascular endothelial cells / 实用口腔医学杂志

ABSTRACT

Objective:To elucidate the effects of Pg in modulation of monocyte adhesion to human umbilical vein endothelial cells (HUVECs).Methods:The model of infection of HUVECs by Pg was established in vitro.HUVECs were infected with Pg381 and Pg33277 and then incubated with THP-1 monocytes respectively.The amount of THP-1 adhesion to HUVECs was tested by rose Ben-gal staining assay.Results:Pg381-infected HUVECs demonstrated significantly increased adhesion of THP-1 cells compared to those infected with Pg33277(0.210 ±0.025 vs 0.078 ±0.024,P0.05).Conclusion:The infection of Pg381 can increased the adhesion of monocytes to HUVECs.