Objective@#To learn the genotypic
drug resistance of men who have sex with men ( MSM ) with
HIV who failed in
antiviral therapy in Yunnan Province, in order to provide basis for improving the effect of
antiviral therapy.@*
Methods@#The
patients who were infected with
HIV-1, homosexual transmitted and failed in
antiviral therapy in Yunnan Province from 2014 to 2019 were recruited. Their
plasma samples were tested by
reverse transcription nested polymerase chain reaction ( RT-
nPCR ) , the fragments were spliced using ContigExpress, and the resistance to 8
protease inhibitors ( PIs ) , 7
nucleoside reverse transcriptase inhibitors ( NRTIs ) and 5 non-
nucleoside reverse transcriptase inhibitors ( NNRTIs ) were obtained from the
HIV drug resistance data
website of Stanford
University.@*Results@#A total of 205
HIV/
AIDS cases were included, 169 positive
plasma samples were amplified, 112 cases were
drug resistant, and the rate of
drug resistance was 66.27%. The
patients who were
aged 30-49 years ( 76.09% ) , had
genotype of CRF01_AE ( 76.34% ) or treated by AZT+3TC+NVP ( 77.08% ) had higher resistance rate. The resistance rates of NNRTIs, NRTIs and PIs were 62.72%, 49.70% and 2.96%, respectively; the resistance rates of NVP and EFV in NNRTIs were 62.72% and 61.54%. The main
mutation site associated with NNRTIs was K103,
accounting for 21.89% ( 37 cases ) ; the main
mutation site associated with NRTIs was M184,
accounting for 39.64% ( 67 cases ) ; the main
mutation sites associated with PIs were M46L/K,
accounting for 2.96% ( 5 cases ) , resulting in high resistance to NFV.@*Conclusions@#The
drug resistance rate of
HIV-infected MSM with failure of
antiviral therapy in Yunnan Province is relatively high, with CRF01_AE as the main
gene subtype of
drug resistance. The
drug resistance rate of NNRTIs is relatively high, especially NVP and EFV.