FTIR-based comparative analysis of glucomannan contents in some tuberous orchids, and effects of pre-processing on glucomannan measurement.

BACKGROUND: Glucomannan (GM) is a polysaccharide of the mannan family of compounds found in some plant species. The dried and powdered tubers of some orchid species, collectively known as 'salep powder,' are a commercially important crop for human consumption and are one of the primary sources of GM. GM content is the primary indicator for the yield and quality of salep powder. We hypothesized that it would be more practical and accurate to measure GM content within tuber powder directly, prior to any purification or pre-processing. The GM content of tubers of 14 different orchid species was evaluated and compared using Fourier transform infrared (FTIR) spectroscopy and an enzymatic colorimetric method. RESULTS: Among the analyzed modes, the sum of the peak areas at 873 and 812 cm-1 , which represent the CH bending attributed to the ß-pyranose form of d-glucose and d-mannose, respectively, gave the only confirmation using colorimetric methods. It was found that the tubers of Himantoglossum caprinum and Serapias vomeracea had the highest GM concentrations among the analyzed species. After conducting different pre-processing steps on Serapias vomeracea tubers, it was found that treating the tubers with milk, or high temperature resulted in an apparent increase in GM concentrations. CONCLUSION: Himantoglossum caprinum and Serapias vomeracea give the highest yields of GM and should be used for commercial horticulture. GM estimation should be made prior to any pre-processing. FTIR spectroscopy is effective and reliable for directly comparing GM content of different orchid species, without the need for any purification or pre-processing. © 2019 Society of Chemical Industry.

Monitoring the effects of chitosan on the profile of certain cell wall and membrane biomolecules in the leaves of Eruca vesicaria ssp. sativa through FT-IR spectroscopy.

The present study aimed to investigate the effects of chitosan at different molecular weights on the biomolecule profile of cell walls and membranes in Eruca vesicaria ssp. sativa leaves through FT-IR spectroscopy. It was demonstrated that the chitosan treatments could increase membrane destabilization through the elevation of lipid peroxidation and/or membrane fluidity. However, 10 kDa chitosan at 5 mg L-1 treatment was estimated to increase membrane lipid production. The 10 and 100 kDa chitosan treatments at 20 mg L-1 suggested higher protein contents than the other treatments. Chitosan's molecular weight and concentration influenced the relative ratios of functional groups in cell wall lignin. Ten kDa chitosan treatments triggered lignin production better than the other chitosan variants. The results showed that its molecular weight plays a role in the differentiation of chitosan's effects on the biomolecule pattern of E. vesicaria ssp. sativa leaves. However, none of the treatments induced significant changes in the peak positions, indicating that ex vitro chitosan treatment did not induce structural changes in the monitored biomolecules. The results also suggested that 10 kDa chitosan at 5 mg L-1 could be a better option than the other treatments tested, considering reducing the chemical use and cost in the cultivation process of the plant.

Characterization of polyphenol oxidase from fennel (Foeniculum vulgare Mill.) seeds as a promising source.

Fennel seeds were recognized as a promising polyphenol oxidase (PPO) source upon investigating some edible green plants (carob, jujube, coriander, fennel, and licorice). The fennel PPO enzyme was purified by three-phase partitioning and biochemically characterized in detail for the first time. The purification fold and activity recovery values were determined as 20-fold and 120%, respectively. Its molecular weight was 27.8 kDa. The temperature for the selected substrates (catechol, 4-tert-butylcatechol, 4-methylcatechol, and pyrogallol) was 30 °C, while the optimum pH value varied from 5.0 to 7.0 depending on the substrate. The kcat/Km values exhibited that the enzyme presented the best activity towards catechol among the substrates used. Sodium metabisulfite, ascorbic acid, benzoic acid, l-cysteine, thiourea, ß-mercaptoethanol, and glutathione prominently inhibited PPO activity. A remarkable decrease in PPO activity was observed at elevated concentrations of organic solvents, but in cases of the solvents with polarity indexes ≥5.1, the residual activity maintained more than 75% of its original activity up to 10% (v/v). Consequently, the current study suggested that fennel seeds could be used in various industrial sectors to produce low-cost polyphenol oxidase enzymes with an agricultural origin.

Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.

This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy. The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves. The chitosan variants enhanced the destabilization of cell membrane structures. CHI-P treatments increased the lipid structure and protein content of the membranes more than CHI-OM treatments. CHI-OM treatment was found to have the ability to induce the formation of ß-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins. CHI-P treatments increased lignin synthesis more than CHI-OM treatments. However, CHI-OM at 10 mg L-1 concentration was more effective than CHI-P treatments in the induction of cell wall polysaccharide synthesis. These findings suggest that the polymerization degree of chitosan plays a role in changing structures and compositions of the biomolecules present in the leaves of I. purpurea.