Halotolerant bacteria isolated from extreme environments induce seed germination and growth of chia (Salvia hispanica L.) and quinoa (Chenopodium quinoa Willd.) under saline stress.

The aim of the study was to characterize halotolerant bacteria and to evaluate their plant growth promotion potential on chia and quinoa seedlings under saline stress. Isolated microorganisms were evaluated for nitrogen fixation, phosphate solubilization, and production of siderophores and indole acetic acid. Three strains and two consortia were selected: Halomonas sp. (SFS), Micrococcus luteus (SA211), Bacillus sp. (HX11), C1 (SA211 + SFS), and C2 (SA211 + HX11). In vitro assays using water agar and half-strength Murashige-Skoog plates showed that an increase in salinity led to an increased seedlings mortality and a decrease in germination (lower than 40%), in total length (varying between 16% and 87% decreases), root length (from 60% to 92% lesser length) and dry weight (from 7% to 86% lower weight). Also, the relative growth index (RGI) decreased for both crops in most treatments, except those with HX11 and C2. These treatments had the highest growth parameters and RGI values in presence of high salinity in chia (50 and 100 mmol/L NaCl) and quinoa (200 and 400 mmol/L NaCl). SA211, the highest producer of indole acetic acid, showed a detrimental effect and anomalous phenotype on plants. Our results suggest that Bacillus sp. HX11, with multiple plant growth promotion traits and tolerance to saline stress, has a great potential as a bioinoculant in saline conditions and could be used as a biofertilizer for crop production.

To burn or not to burn: The question of straw burning and nitrogen fertilization effect on nitrous oxide emissions in sugarcane.

Nitrous oxide (N2O) is the main greenhouse gas emitted from farming systems and is associated with nitrogen (N) fertilizer application as well as decomposition of organic matter present in the environment. The objective of this study was to determine the effect of post-harvest straw burning and synthetic N fertilization on the dynamics of N2O emissions in the sugarcane-soil system in Tucuman, Argentina, compared with a native forest. Close-vented chambers were used to capture N2O during three consecutive growing seasons. The highest N2O emissions from the sugarcane-soil system coincided with the period of high soil and air temperatures, rainfall and soil N content. The effect of synthetic N fertilization on annual cumulative N2O emission was 7.4-61.5% higher in straw burned than in unburned treatments, especially during a wet growing season. There was a significant effect of treatments on N2O emission factors among growing seasons: 0.58-1.67% and 0.94-3.34% in the unburnt and burnt treatments, respectively. The emission factors for sugarcane are highly dependent on rainfall, temperature and crop management practices; regarding the latter, avoiding straw burning and reducing N soil availability, assessing alternative N fertilizers or new application modes such as split rates, seem to be the key for mitigating N2O emissions from the sugarcane-soil system in Tucumán, Argentina.