RESUMEN
The etiological structure of influenza and other acute respiratory viral infections including their rate of incidence in St. Petersburg and Leningrad region during 4 epidemic seasons has been studied. Seasonality of some respiratory viruses was shown and peaks of circulation of RSV, adenovirus, parainfluenza viruses, rhinovirus, bocavirus, metapneumovirus and coronavirus were marked. The interference of influenza A viruses and RSV, RSV and rhinoviruses was highlighted. A high incidence of adenovirus infection in organized communities and RSV infection in children was revealed.
Asunto(s)
Infecciones por Adenovirus Humanos/epidemiología , Gripe Humana/epidemiología , Infecciones por Paramyxoviridae/epidemiología , Infecciones del Sistema Respiratorio/epidemiología , Adenoviridae/patogenicidad , Infecciones por Adenovirus Humanos/virología , Adolescente , Bocavirus/patogenicidad , Niño , Coronavirus/patogenicidad , Epidemias , Humanos , Lactante , Virus de la Influenza A/patogenicidad , Gripe Humana/virología , Metapneumovirus/patogenicidad , Infecciones por Paramyxoviridae/virología , Virus Sincitiales Respiratorios/clasificación , Virus Sincitiales Respiratorios/patogenicidad , Infecciones del Sistema Respiratorio/virología , Rhinovirus/patogenicidad , Federación de Rusia/epidemiología , Estaciones del AñoRESUMEN
Antigenic and genetic characteristics of Russian RSV isolates are presented for the first time. Of the 69 strains isolated in St. Petersburg, 93% belonged to the RSV-A antigenic group. The antigenic variations in the F-protein RSV were analyzed using a panel from 6 monoclonal antibodies by the method of micro-cultural ELISA. Depending on the decrease in the effectiveness of interaction with monoclonal antibodies (relative to the reference strain Long), RSV-A isolates were divided into 4 antigenic subgroups. The results of 24 isolates sequencing showed that more than 60% of them had substitutions in significant F-protein sites compared to the ON67-1210A reference strain of the current RSV genotype ON1/GA2. The most variable were the signal peptide and antigenic site II. When comparing the results of ELISA and sequencing, it was not possible to identify any specific key substitutions in the amino acid sequence of the F-protein that affect the interaction of the virus with antibodies. The nucleotide sequence of the F-gene from 19 of the 24 characterized isolates was close to that of ON67-1210A reference virus and was significantly different from RSV-A Long and A2 viruses. A separate group consisted of 5 strains, in which the F-protein structure was approximated to RSV Long.