Effect of common horsetail extract on growth characteristics, essential oil yield and chemical compositions of basil (Ocimum basilicum L.).

To investigate the effect of horsetail extract containing high silicon on morphological traits, growth, content, and compositions of essential oil of sweet basil (Ocimum basilicum L.) an experiment turned into carried out in the shape of a randomized complete block design with three replications. Foliar treatment of horsetail extract with zero, 0.5, 1, and 2% concentrations was applied on 6-8 leaf plants. The assessed traits include plant height, number of leaves per plant, number of sub-branches, leaf area index, plant fresh weight, plant dry weight, total anthocyanin, the content of total phenol and total flavonoid, antioxidant activity, essential oil content, and compounds were measured. The findings demonstrated that the increase of silicon-containing horsetail extract enhanced the improved increase in growth and phytochemical trait values. The use of horsetail extract in the 2% treatment increased plant height, the number of leaves per plant, the number of sub-branches, leaf area index, fresh weight, and dry weight of the plant by 49.79, 45.61, 91.09, 99.78, 52.78 and 109.25%, respectively, compared to the control. The highest content of total phenol (2.12 mg GAE/g DW), total flavonoid (1.73 mg RE/g DW), total anthocyanin (0.83 mg C3G/g DW), and antioxidant activity (184.3 µg/ml) was observed in the 2% extract treatment. The content of essential oil increased with increasing the concentration of horsetail extract, so the highest amount of essential oil was obtained at the concentration of 2%, which increased by 134.78% compared to the control. By using GC-MS, the essential oil was analyzed. The main components of the essential oil include methyl eugenol (12.93-25.93%), eugenol (17.63-27.51%), 1,8-cineole (15.63-20.84%), linalool (8.31-19.63%) and (Z)-caryophyllene (6.02-14.93%). Increasing the concentration of horsetail extract increased the compounds of eugenol, 1,8-cineole, and linalool in essential oil compared to the control, but decreased the compounds of methyl eugenol and (Z)-caryophyllene. Foliar spraying of horsetail extract, which contains high amounts of silicon, as a stimulant and biological fertilizer, can be a beneficial ingredient in increasing the yield and production of medicinal plants, especially in organic essential oil production.

Enhancing lucerne (Medicago sativa) yield and nutritional quality: a meta-analysis of fertilization types and environmental factors in China.

Introduction: Lucerne (Medicago sativa), is a cornerstone of China's livestock industry, however, due to the backward agronomic strategies and technology, lucerne in China faces cultivation challenges that result in lower productivity and quality than global standards. Therefore, we undertook a meta-analysis to evaluate the impact of five distinct fertilization types on lucerne yield and nutritional quality in various locations in China. The fertilizer practices included manure application, combined mineral fertilizer and manure application (FM), biological fertilizer application, unbalanced application of two or more mineral fertilizer types, and balanced mineral fertilizer application. Furthermore, we investigate influential factors of yield and quality of lucerne under fertilization, including climatic variables (mean annual precipitation, mean annual temperature), initial soil properties (soil organic carbon; total nitrogen, pH), and agronomic factors (seeding rate, harvest frequency, and lucerne stand age). Methods: Our study analyzed 53 published papers to discern the most beneficial fertilizer for enhancing lucerne yield and nutritional quality. Results and discussion: The results showed that the fertilizer practices, on average, significantly improved yield by 31.72% and crude protein content by 11.29%, with FM emerging as the most effective, this is because mineral fertilizers provide available nutrients for lucerne, manure provides essential organic matter for microorganisms and improve soil properties. In addition, the fertilizer practices significantly reduced neutral and acid detergent fiber contents by 6.28% and 8.50%, respectively, while increasing ash content and relative feeding value. Furthermore, climatic variables, soil properties, and planting system factors such as sowing date and harvest frequency significantly affected yield and nutritional quality. The practical implications of our results emphasize the need for balanced and strategic fertilizer application to optimize lucerne production and highlight the potential to adjust cultivation practices according to environmental conditions. Balanced and strategic fertilizer application can simultaneously improve soil properties, enhance soil carbon sequestration, and reduce the emission of greenhouse gases from the soil, which is a vital measure for realizing sustainable agricultural development.